. Author manuscript; available in PMC: 2020 Mar 1.

Published in final edited form as: Exp Eye Res. 2018 Dec 14;180:86–91. doi: 10.1016/j.exer.2018.12.003

Table 1.

PCR primers used for SLC4A11 Mutagenesis.

Mutation	Primers (5’ -> 3’)
R125H	Forward: AACTTCAAGGAAGAGATCCATGCGCACCGCGACCTAGAT
R125H	Reverse: ATCTAGGTCGCGGTGCGCATGGATCTCTTCCTTGAAGTT

W240S	Forward: CACAGAACTCGGGGGAGAATTCCTGTGAGGTTC
W240S	Reverse: GAACCTCACAGGAATTCTCCCCCGAGTTCTGTG

C386R	Forward: CTGTTCCTCTACTTCGCCCGCCTCCTG
C386R	Reverse: CAGGAGGCGGGCGAAGTAGAGGAACAG

V507I	Forward: CATCACGTTTGTGCTGGATGCCATCAAGGGCAC
V507I	Reverse: GTGCCCTTGATGGCATCCAGCACAAACGTGATG

N639A	Forward: GATGAGCAAGTTCCGCTACGCCCCCAGCGAGAG
N639A	Reverse: CTCTCGCTGGGGGCGTAGCGGAACTTGCTCATC

Mutated nucleotides were indicated by underline.