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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Exp Eye Res. 2018 Dec 10;180:63–74. doi: 10.1016/j.exer.2018.11.020

Figure 1. Loss of IR expression in the RPE of RPEIRKO mice.

Figure 1.

(A) RPE/choroid flatmounts were prepared from non-diabetic IR flox and RPEIRKO mice at 6 weeks of age. Tissue was stained with mouse anti-Cre antibody (green), rabbit anti-IRβ antibody (red) and counterstained with DAPI. Scale bar = 500μm. (B) RPE lysates were prepared from IR flox, Best1-Cre and RPEIRKO mice. Western blotting was performed using anti-IRβ, anti-actin (top) and anti-cre recombinase (bottom) antibodies.