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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Mol Biol. 2019 Jan 15;431(5):970–980. doi: 10.1016/j.jmb.2019.01.013

Fig. 3. Bioinformatic analysis predicts 14 additional FMN-binding GUS enzymes from the human gut microbiota.

Fig. 3.

(A) SSN of the HMP GUSome with putative FMN-binders as larger, outlined circles and those that were synthesized and characterized further are labeled. BLAST/e-value/cut off value utilized to generate this SSN is 10−220 and sequences were acquired from the HMP stool sample database. (B) Genome neighborhood diagrams of putative FMN-binding GUSs (C) Absorbance scans of purified Rh2GUS, Rg3GUS, BvGUS, and RiGUS reveal flavin binding profile. (D) Mass spectra of purified Rh2GUS, Rg3GUS, BvGUS, and RiGUS reveal mass for FMN.