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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Mol Biol. 2019 Jan 15;431(5):970–980. doi: 10.1016/j.jmb.2019.01.013

Fig. 4. Binding studies and structural analysis demonstrate that GUS enzymes utilize a conserved motif to bind FMN.

Fig. 4.

(A) Binding affinities (Kd) of GUS enzymes and FMN-binding site mutants for FMN as determined by isothermal titration calorimetry (B) Structures of FMN-binding sites as determined by crystallography for Rg3GUS (blue) and Rh2GUS (magenta) and by template-guided modeling for BvGUS (orange) and RiGUS (green).