KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ABCB1 | Cell signaling | Cat#12683S; RRID:AB_2715689 |
| ABCG2 | Cell signaling | Cat#4477S; RRID: AB_10544928 |
| CDK12 | Cell signaling | Cat#11973S; RRID:AB_2715688 |
| MYCN | Cell signaling | Cat#9405S; RRID:AB_10692664 |
| c-MYC | Santa Cruz | Cat#sc-40; RRID:AB_627268 |
| Cleaved PARP | Cell signaling | Cat#9541S; RRID:AB_331426 |
| GAPDH | Cell signaling | Cat#2118; RRID:AB_561053 |
| MCL1 | Santa Cruz | Cat#sc-819; RRID:AB_2144105 |
| CDK7 | Santa Cruz | Cat#sc-723; RRID:AB_2077155 |
| RNAPII | Santa Cruz | Cat#sc-899; RRID:AB_632359 |
| Phospho-RNAPII S2 | Bethyl | Cat#300–654A; RRID:AB_519341 |
| Phospho-RNAPII S5 | Bethyl | Cat#A304–408A; RRID:AB_2620602 |
| Phospho-RNAPII S7 | Millipore | Cat#04–1570; RRID:AB_10618152 |
| Bacterial and Virus Strains | ||
| TRCN0000059683: | Sigma-Aldrich | Clone ID:NM_000927.3–3500s1c1 |
| TRCN0000059684 | Sigma-Aldrich | Clone ID:NM_000927.3–1193s1c1 |
| TRCN0000059800 | Sigma-Aldrich | Clone ID:NM_004827.1–1718s1c1 |
| TRCN0000059802 | Sigma-Aldrich | Clone ID:NM_004827.1–544s1c1 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tariquidar | Selleck | Cat#S8028; CAS:206873–63-4 |
| KO-143 | Sigma-Aldrich | Cat#K2144; CAS:461054–93-3 |
| THZ1 | Gray Lab (Kwiatkowski et al., 2014) | CAS:1604810–83-4 |
| THZ531 | Gray Lab (Zhang et al., 2016) | CAS:1702809–17-3 |
| AT7519 | MedChem Express | Cat#HY-50943; CAS:844442–38-2 |
| SNS-032 | Selleck | Cat#S1145; CAS:345627–80-7 |
| AZD5438 | ChemExpress | Cat#HY-10008; CAS:602306–29-6 |
| CR8 | Sigma-Aldrich | Cat#C3249; CAS:294646–77-8 |
| Dinaciclib | ChemExpress | Cat#HY-10492; CAS:779353–01-4 |
| E9 | This study | N/A |
| E9-R | This study | N/A |
| Flavopiridol | ChemExpress | Cat#HY-10005; CAS:146426–40-6 |
| LDC000067 | ChemExpress | Cat#HY-15878; CAS:1073485–20-7 |
| Ly2857785 | ChemExpress | Cat#HY-12293; CAS:1619903–54-6 |
| NVP2 | Gray Lab | N/A |
| P276–00 | Selleck | Cat#S8058; CAS:920113–03-7 |
| PD-0332991 | ChemExpress | Cat#HY-50767; CAS:827022–33-3 |
| PHA-848125 | Selleck | Cat#S2751; CAS:802539–81 −7 |
| R547 | ChemExpress | Cat#HY-10014; CAS:741713–40-6 |
| Ribociclib | ChemExpress | Cat#HY-15777B; CAS:1211441–98-3 |
| RO-3306 | ChemExpress | Cat#HY-12529; CAS:872573–93-8 |
| Critical Commercial Assays | ||
| DC protein Assay | Bio-Rad | Cat#5000111 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat#G7573 |
| SuperScript III Reverse Transcriptase | Thermo Fisher | Cat#18080044 |
| RNeasy Mini Kit | Qiagen | Cat#74106 |
| QIAamp DNA mini Kit | Qiagen | Cat#51304 |
| Experimental Models: Cell Lines | ||
| Human: Kelly | Children’s Oncology Group | N/A |
| Human: LAN5 | Children’s Oncology Group | N/A |
| Human: SK-N-BE2 | Children’s Oncology Group | N/A |
| Human: NGP | Children’s Oncology Group | N/A |
| Human: PC9 | ATCC | N/A |
| Human: H3122 | NCI Tumor Cell Line Repository | N/A |
| Human: NCI-H82 | ATCC | ATCC® HTB-175™ |
| Human: HAP1 WT & transformed | Gray Lab (Zhang et al., 2016) | N/A |
| Oligonucleotides | ||
| RT-qPCR primer: ABCB1 Forward: TGACATTTATTCAAAGTTAAAAGCA |
This paper | N/A |
| RT-qPCR primer: ABCB1 Reverse: TGACATTTATTCAAAGTTAAAAGCA |
This paper | N/A |
| RT-qPCR primer: ABCG2 Forward: CTTCTTCCTGACGACCAACC |
This paper | N/A |
| RT-qPCR primer: ABCG2 Reverse: TCTGTAGTATCCGCTGATGTATTC |
This paper | N/A |
| RT-qPCR primer: ABCC1 Forward: AATACCAGCAACCCCGACTTCAC |
This paper | N/A |
| RT-qPCR primer: ABCC1 Reverse: GGTGTCATCTGAATGTAGCC |
This paper | N/A |
| RT-qPCR primers for ABCC3, ABCC4, CDK7, GAPDH, genomic qPCR primers for ABCB1, ABCG2 and CDK12, and sequencing primers for CDK7, CDK12 and CDK13, see Table S2. |
This paper | N/A |
| Software and Algorithms | ||
| FlowJo 10 | FlowJo, LLC | https://www.flowjo.com/solutions/flowjo |
| GraphPad Prism 7.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |