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. 2019 Feb 19;10:91. doi: 10.3389/fendo.2019.00091

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Copyright © 2019 Guo, Liu, Zhao, Mi, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combined effects of FSH and SCF on cell apoptosis. (A) Ovarian cells (arrowheads) with TUNEL (green) marker indicate cell apoptosis after the treatment of 4-day-old chicken ovaries with FSH or SCF in vitro for 3 days. DAPI (blue) represents nuclear DNA. Scale bar: 30 μm. (B) The percentage of TUNEL positive cells reduced significantly after FSH and SCF treatment. (C) Caspase-3 protein is indicated by a brown stain. Arrowheads represent the ovarian cells stained caspased-3 marker. (D,E) Western blot and gray analysis indicate that FSH inhibited caspase-3 protein expression. SCF acted to enhance the effect of FSH in inhibiting caspase-3 expression in vitro. T-tests were used to determine statistically significant differences. The values are the mean ± SEM of six experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001).