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. 2019 Feb 19;10:91. doi: 10.3389/fendo.2019.00091

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Copyright © 2019 Guo, Liu, Zhao, Mi, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of SCF mRNA in the chicken ovaries. SCF mRNA showed staining in the cell cytoplasm by in situ hybridization. (A) SCF mRNA was detected in the cytoplasm in 6-day-old chicken ovarian cells. Arrows and arrowheads represent the cysts and follicles, respectively. (B) SCF mRNA expression was measured by qRT-PCR in the chicken ovaries from Day 1 to 9. GAPDH was used as the normalization control. T-tests were used to determine statistically significant differences. The values are the mean ± SEM of six experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01).