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. 2019 Feb 19;10:91. doi: 10.3389/fendo.2019.00091

Figure 6.

Figure 6

SCF enhanced the stimulatory effect of FSH in vitro. The 4-day-old chicken ovaries were treated with FSH and SCF for 2 days in culture. (A,B) Western blot and gray analysis indicate that SCF enhanced the effect of FSH on promoting c-KIT and FSHR protein expressions. (E) qRT-PCR analysis indicates that SCF could enhance the effect of FSH to promote FSHR mRNA expression. Western blot and gray analysis indicate that SCF could enhance the effect of FSH to increase ratio of p-AKT/AKT (A,C) and ratio of p-MAPK/MAPK (A,D). (F) FSH also combined with SCF to promote TAF4b and Nobox mRNA expression. T-tests were used to determine statistically significant differences. The values are the mean ± SEM of six experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001).