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. 2019 Feb 19;10:91. doi: 10.3389/fendo.2019.00091

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Copyright © 2019 Guo, Liu, Zhao, Mi, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combined effects of FSH and SCF on cell proliferation. (A) The 6th day chicken ovaries were incubated for 6 h with BrdU after treatment by FSH in vivo. Arrowheads represent ovarian cells with BrdU (red) marker in the ovarian cortex. DAPI (blue) represents the nuclear DNA. Scale bar: 20 μm. (B) The percentage of BrdU positive cells increased significantly after FSH treatment. (C,D) Western blot and gray analysis indicate that FSH promoted PCNA protein expression. (E) 4-day-old chicken ovaries were treated by FSH or SCF in vitro for 3 days. Arrowheads represent the cells with BrdU (red) marker. DAPI (blue) represents nuclear DNA. Scale bar: 30 μm. (G) The percentage of BrdU positive cells increased significantly with FSH and SCF treatment in combination. (F,H) SCF enhanced the effect of FSH to promote the PCNA protein expression. T-tests were used to determine statistically significant differences. The values are the mean ± SEM of six experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001).