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. 2019 Feb 19;10:22. doi: 10.3389/fgene.2019.00022

FIGURE 2.

FIGURE 2

Effects of miR-378a-5p on the proliferation and migration in vascular smooth muscle cell (VSMC). (A) Expression levels of miR-378a-5p were examined by qRT-PCR after transfection of miR-378a-5p mimics, miR-378a-5p inhibitor and negative control oligonucleotide (NC), ∗∗∗p < 0.001. (B) The CCK-8 assay was performed to investigate the proliferation of VSMCs with miR-378a-5p mimics, miR-378a-5p inhibitor transfection, at 0, 12, 24, and 36 h, respectively. (C) Representative micrographs of EdU staining of VSMCs, with control or miR-378a-5p transfection, scale bar = 20 μm. (D,E) miR-378a-5p effects on VSMC migration ability as determined via classic scratch assay and its quantification analysis, all images were taken under the same magnification, data are presented as mean ± SEM, ∗p < 0.05 and ∗∗p < 0.01.