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. 2019 Feb 19;10:22. doi: 10.3389/fgene.2019.00022

FIGURE 3.

FIGURE 3

MiR-378a-5p promotes platelet-derived growth factor-BB (PDGF-BB)-induced VSMC proliferation and migration. (A) miR-378a-5p expression levels were increased in VSMCs under the time gradient of PDGF-BB-induced (50 ng/ml), compared with quiescent cells, as demonstrated using qRT-PCR, data are presented as mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01. (B,C) miR-378a-5p abrogated PDGF-BB-mediated effects on VSMC migration ability as determined via classic scratch assay and its quantification analysis, all images were taken under the same magnification. (D,E) The miR-378a-5p mimics significantly increased PDGF-BB-induced (50 ng/ml) VSMC migration, as determined by transwell assay (original magnification: × 200) and its quantification analysis, data are presented as mean ± SEM. ∗p < 0.05. (F) Representative western blots of VSMC phenotype marker genes transfected with miR-378a-5p inhibitor compared with in NC under the condition with PDGF-BB (50 ng/ml) stimulated for 12 h. (G) Quantitative analysis of differentiation marker gene expression in the two groups, data are presented as mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01. (H) Representative western blots of VSMC phenotype marker genes transfected with miR-378a-5p mimics compared with NC under the condition with PDGF-BB (50 ng/ml) stimulated for 12 h. (I) Quantitative analysis of differentiation marker gene expression in the two groups, data are presented as mean ± SEM. ∗p < 0.05.