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. 2019 Feb 19;10:22. doi: 10.3389/fgene.2019.00022

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Copyright © 2019 Liu, Yang, Jiang, Xu, Tang, Lobo, Zhang, Liu, Yu and Xin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-378a-5p participates in the regulation of CDK1 expression. (A) There is a potential seed sequence of miR-378a-5p in the 3′UTR of CDK1, the putative seed sequences for the 3′-UTR of CDK1 were highly conserved in different species. (B) qRT-PCR, (C,D) western blot were used to determine CDK1 mRNA and protein expression levels and p21 protein levels in VSMCs transfected with miR-378a-5p mimics (25 nmol/L), inhibitor (100 nmol/L), NC (25 nmol/L) and inNC (25 nmol/L) after PDGF-BB stimulation, data are presented as mean ± SEM, ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. (E) Typical fluorescence photomicrograph by laser scan confocal microscopy, scale bar = 20 μm. The photographs of nuclei (blue) and CDK1 (green) fluorescence were taken under a same field and then were merged. (F) Real-time PCR validation of CDK1-associated mRNAs identified by RIP, data are presented as mean ± SEM. ^∗∗p < 0.01.