Table 1.

Repeated measures ANOVA for the effect of saline stress (S) and microbial symbiosis (Sy) on two variables of physiological performance (a: photosynthetic efficiency; b: lipid peroxidation) and two specific stress-response mechanisms (c: proline concentration; d: NHX1 relative expression).

Response variable	Source of variation	dfnum	dfden	F	p
Photochemical efficiency (Fv/Fm)	Stress (S)	1	72	908445.8	<0.0001
	Symbiont (Sy)	1	36	1226.8	<0.0001
	Time (T)	1	36	547.8	<0.0001
	Stress × Symbiont	2	72	366.3	<0.0001
	Stress × Time	1	36	149.1	<0.0001
	Symbiont × Time	2	72	396.4	<0.0001
	S × Sy × T	2	72	165.6	<0.0001
Lipid peroxidation (TBARS [MDA])	Stress (S)	1	32	16724.88	<0.0001
	Symbiont (Sy)	1	16	816.535	<0.0001
	Time (T)	1	16	382.987	<0.0001
	Stress × Symbiont	2	32	487.53	<0.0001
	Stress × Time	1	16	330.013	<0.0001
	Symbiont × Time	2	32	452.058	<0.0001
	S × Sy × T	2	32	207.78	<0.0001
Proline concentration (mmol/g FW)	Stress (S)	1	16	901.146	<0.0001
	Symbiont (Sy)	1	16	137.093	<0.0001
	Time (T)	2	32	185.671	<0.0001
	Stress × Symbiont	1	16	117.031	<0.0001
	Stress × Time	2	32	171.692	<0.0001
	Symbiont × Time	2	32	30.934	<0.0001
	S × Sy × T	2	32	29.946	<0.0001
NHX1 gene expression (Relative fold change)	Stress (S)	1	32	513.41	<0.0001
	Symbiont (Sy)	1	16	90.69	<0.0001
	Time (T)	1	16	1065.19	<0.0001
	Stress × Symbiont	2	3.2	23.23	0.0002
	Stress × Time	1	16	755.79	<0.0001
	Symbiont × Time	2	32	92.31	<0.0001
	S × Sy × T	2	32	31.47	<0.0001

The table shows the probability value (p) obtained for the significance of each factor in the fitted linear mixed model, which was obtained by maximizing the restricted log-likelihood for the data.