Figure 5.

Loss of JAK1 inhibits tumor cell apoptosis. B16/OVA cells and their derivatives were stimulated with mIFN-γ (100 ng/mL) or mIFN-α4 (500 ng/mL) for 72 h. (A,B) Cleaved caspase-3 expression was analyzed by flow cytometry 24 h later. (C,D) Remaining live cells were counted with a hemocytometer after 72 h. (E,F) B16F10 and MC38 with JAK1 were stimulated with mIFN-α4 (500 ng/mL) for 72 h and the remaining live cells were counted. Data are presented as mean ± SEM. ^**P < 0.01 vs. B16/OVA/B16F10/MC38 control group; ^##P < 0.01 vs. B16/OVA/B16F10/MC38 + IFN-α group.