Table 1.
Oligonucleotide Primers.
| No. | Name | Protein sequence | Sequence |
|---|---|---|---|
| 4012 | C-lobefor | GSSSENK | GCGCGGATCCTCGTCTGAAAACAGTAAGCTG |
| 3181 | C-loberev | KRQQPVQ* | ATGCCAGTAAGCTTTTATTGCACAGGCTGTTGGCGTTTC |
| 4046 | LCLlp1rev | VDGGIMIDLAG | TCCGGCCAGATCAATCATAATGCCGTCGAC |
| 4045 | LCLlp1for | ILDLAGTEFTR | ATTGATCTGGCCGGAACAGAATTTACCCGC |
| 4048 | LCLlp2rev | RKFEHTTINGK | TTTGCCGTTAATCGTGTGTTCAAATTTGCG |
| 4047 | LCLlp2for | TINGKTYEVE | ACGATTAACGGCAAAACCTATGAAGTCGAA |
| 4050 | LCLlp3rev | GMLTRKGKQV | AACCTGTTTGCCTTTGCGCGTCAACATTCC |
| 4049 | LCLlp3for | KGKQVEQSMF | AAAGGCAAACAGGTTGAACAAAGTATGTTC |
| 4052 | LCLlp4rev | GGWFAYHKSDNGS | GCTGCCGTTATCGCTTTTATGATAGGCAAACCATCCGCC |
| 4151 | LCLlp4for | YHKSDNGSATVVF | TATCATAAAAGCGATAACGGCAGCGCGACCGTGGTATTC |
| 4157 | LCLlp1-helixrev | IPLLPATKAVFDENRK | ATTTGCGGTTCTCATCAAAGACGGCTTTTGTGGCTGGGAGGAGTGGAAT |
| 4156 | LCLlp1-helixfor | AVFDENRKYGPEFTRKFE | GCCGTCTTTGATGAGAACCGCAAATACGGCCCGGAATTTACCCGCAAATTTGAA |
| 4159 | LCLlp2-lp10rev | TRKFEHTAKEITELLGSRALLNGNSRN | ATTGCGGCTGTTGCCGTTGAGCAAAGCCCGGCTGCCCAACAACTCTGTGATTTCCTTGGCCGTGTGTTCAAATTTGCGGGT |
| 4158 | LCLlp2-lp10for | SRALLNGNSRNTKATARRTRTYEVEVC | AGCCGGGCTTTGCTCAACGGCAACAGCCGCAATACAAAAGCCACCGCGCGCCGTACCCGCACCTATGAAGTCGAAGTCTGC |
| 4161 | LCLlp3-Lp11rev | YGMLTRKVTWENVRQTAGGAVNQHK | TTGTGTTGGTTGACTGCGCCGCCGGCAGTTTGCCGCACATTTTCCCAAGTAACTTTGCGCGTCAACATTCCGTA |
| 4160 | LCLlp3-Lp11for | AGGAVNQHKNVGVYNRYAVEQSMFL | GCCGGCGGCGCAGTCAACCAACACAAAAATGTCGGCGTTTACAACCGATATGCCGTTGAACAAAGTATGTTCCTC |
| 4163 | LCLlp4-pluglprev | LGGWFAYTAQAALGGTRT | CGTCCTCGTCCCGCCCAATGCCGCCTGCGCGGTATAGGCAAACCATCCGCCCAA |
| 4162 | LCLlp4-pluglpfor | AALGGTRTAGSSATVV-FG | GCGGCATTGGGCGGGACGAGGACGGCGGGCAGCAGCGCGACCGTGGTATTCGGT |
| 4755 | HpTbpBfor | GSGVSKE | TGAACAGGATCCGGCGTGTCTAAAGAAG |
| 4756 | HpTbpBrev | GAKQQVKK | TGTTCAACTAGTTTATTTTTTTACTTGTTGTTTTGCACC |
| 4772 | RvsPos4uplp10 | LGGQFRYQKAKE | CCTTGGCTTTTTGATAACGGAATTGTCCGCCAA |
| 4773 | FwdPos4uplp10 | GQFRYQKAKEITELLG | GACAATTCCGTTATCAAAAAGCCAAGGAAATCACAGAGTTGTTGGG |
| 4774 | Revlp10Pos4dn | ARRTRPVGVGAVFGAKQQ | CTTGTTGTTTTGCACCAAAGACAGCACCTACACCTACAGGGCGGGTACGGCGCG |
| TbpBKO_UpF | ATGACGCGATTAGAGTTTCA | ||
| TbpBKO_UpR | MNNPLVI (TbpB) | GGATATCAGCTGGATGGCAATCACCAATGGATTGTTCATA | |
| TbpBKO_DownF | TAATTGGTTGTAACACTGGCAGCACGGCTGCCGAACAATC | ||
| TbpBKO_DownR | YSIRGMD (TbpA) | CCATACCGCGTATCGAGTAG | |
| 4600 | Kan_F | TTGCCATCCAGCTGATATCC | |
| 4601 | Kan_R | GCCAGTGTTACAACCAATTA |
*
Underlined residues are sequences of the inserted loops.