Figure 3.

Percentage changes in Neuro2a- and C6-cell viability after 12 h and 24 h treatment with different doses of NA and H₂O₂ are shown. Viability of both Neuro2a (A) and C6 (B) was least affected (<15% of control) by treatment with up to 10 μM of NA, whereas H₂O₂ affected the cell viability in even much lesser concentration. *p < 0.05, **p < 0.01, ***p < 0.001 as compared to control. Abbreviations are as in the text. (C) Representative microscopic fields of images of Neuro2a and C6 cells with or without treatment with NA (10 μM), H₂O₂ or their combination are shown. Both Neuro2a as well as C6 cells showed less adherence to the petri-dish. Those remained adhered, showed membrane granulation and more circular (marked by arrows as representative), which are signs of cellular stress/damage or approaching death upon H₂O₂ treatment for 24 h. Cells treated with H₂O₂ in presence of NA showed reduced -detachment, -granulation and -circular shaped in addition to lesser death. Scale bar 100 μm.