Figure 5.

NA mediated modulation of intracellular ROS levels in presence of iron in Neuro2a (A) and C6 (B) is shown. 2 × 10⁵ cells per well were seeded in 96 well plates and incubated for 12 h in serum free medium lacking iron or in medium containing 10 μM FeSO₄ as source of iron (Fe²⁺). Cells in both the media were treated with H₂O₂ alone or in presence of NA or DFX for 2 h. At the end the cells were incubated with medium containing 25 μM DCFDA for 30 min followed by PBS washing and DCF fluorescence estimation. In the presence of Fe²⁺, H₂O₂ enhanced ROS generation in both the cells, Neuro2a (A) and C6 (B), which were prevented by DFX and NA. **p < 0.01, ***p < 0.001 as compared with control. Abbreviations are in the text.