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. 2019 Feb 19;10:255. doi: 10.3389/fimmu.2019.00255

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Copyright © 2019 Katakura, Nishiya, Wentzel, Hino, Miyamae, Okano, Wiegertjes and Moritomo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Administration of G-CSFa1 and G-CSFb1 to carp increases circulating blood neutrophil population. Peripheral blood leukocytes were collected from carp 2 days before and 6 and 24 h after intraperitoneal injection of 1 × PBS, recombinant G-CSFa1 and G-CSFb1 or of unhandled carp and analyzed by flow cytometry. Percentage of gated neutrophil population per live peripheral blood leukocytes was measured. Three fish for each group were used and data are presented as mean + standard deviation. Significant differences compared to other treatment groups and other time points were determined using two-way ANOVA followed by Tukey's post-hoc test, (p < 0.05) are denoted by different letters.