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. 2019 Feb 19;10:255. doi: 10.3389/fimmu.2019.00255

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Copyright © 2019 Katakura, Nishiya, Wentzel, Hino, Miyamae, Okano, Wiegertjes and Moritomo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative mRNA expression analysis of carp g-csf paralogs in carp tissues. Basal gene expression of carp G-CSF paralogs in spleen, muscle, head kidney, trunk kidney, gill, brain, thymus, skin, liver, gut, and heart. Basal expression levels were determined relative to the s11 protein of the 40s subunit (40s) as a reference gene and are presented as mean + standard deviation (n = 3, except thymus, n =2). G-csfb1 expression was non-detectable in all tissues examined (ND indicates “non-detectable”). Significant differences in expression between tissues were determined using one-way ANOVA followed by Tukey's HSD (g-csfa1 and g-csfb2) or Dunnet's T3 post-hoc test for unequal variances (g-csfa2).