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. 2019 Feb 19;10:255. doi: 10.3389/fimmu.2019.00255

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Copyright © 2019 Katakura, Nishiya, Wentzel, Hino, Miyamae, Okano, Wiegertjes and Moritomo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative mRNA expression analysis of carp g-csf paralogs in immune cells. Basal gene expression of carp g-csfa1 and g-csfa2 (A) and g-csfb1 and g-csfb2 (B) in sorted cells from healthy carp. Basal expression levels were determined relative to the s11 protein of the 40s subunit (40s) as a reference gene and presented as mean + standard deviation (n = 4 except thymocytes and thrombocytes n = 2). Statistical significance within the macrophage group was determined using an un-paired Student's t-test. Asterisks (^*) denotes significance (p < 0.05) between indicated genes. ND indicates “non-detectable”.