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. 2019 Feb 19;10:255. doi: 10.3389/fimmu.2019.00255

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Copyright © 2019 Katakura, Nishiya, Wentzel, Hino, Miyamae, Okano, Wiegertjes and Moritomo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant G-CSFa1 and G-CSFb1 induces increased respiratory burst capability. Respiratory burst capability of kidney neutrophils after pre-treatment with the medium, 100 ng/mL G-CSFa1 or 100 ng/mL G-CSFb1 for 6 h and subsequently treated with or without 100 ng/mL PMA for 30 min in the presence of DHR123. Mean of DHR123 fluorescence intensity (MFI) in gated neutrophil population was measured by flow cytometry. Data points are presented as mean values of individuals and error bars show standard deviation. Kidney neutrophils were obtained from four fish. Significant differences compared to every other group with two factors of G-CSF pre-treatment and PMA treatment were determined using two-way ANOVA followed by Tukey's post-hoc test, (p < 0.05) are denoted by asterisks (^*). N.S. represents ‘not significant’.