Table 1.

The number of type 1 and type 2 colonies formed from 100,000 cells in head kidney, trunk kidney, spleen, and PBLs in the semi-solid culture with the combination of 100 ng/mL G-CSFa1 and 100 ng/mL G-CSFb1.

	Head kidney		Trunk kidney		Spleen		PBLs
	Type 1 (CFU-G)	Type 2 (CFU-GM)	Type 1 (CFU-G)	Type 2 (CFU-GM)	Type 1 (CFU-G)	Type 2 (CFU-GM)	Type 1 (CFU-G)	Type 2 (CFU-GM)
Mean of colonies formed in the presence of both G-CSFa1 and G-CSFb1 (± SEM)	29.1 ± 4.2	34.5 ± 4.5	22.9 ± 3.5	34.0 ± 3.8	0	0	0	0
Mean of leukocytes in each whole organ	9.24 × 106		8.94 × 106		3.60 × 105		ND
No. of progenitors estimated (± SEM)	2,689 ± 388	3,188 ± 416	2,047 ± 313	3,039 ± 340	0	0	0	0

Data were obtained from duplicate cultures in the presence or absence of both 100 ng/mL G-CSFa1 and 100 ng/mL G-CSFb1 and shown only from the culture with the cytokines (n = 4). Type 1 colonies were counted at 7 days of cultivation. Type 2 colonies were counted at 10 days of cultivation. ND, not determined.