Table 3.
Effect of mother tree and controlled crosses on the frequency of maritime pine megagametophytes that produced established EMs after 4 months in culture.
| Year | Mother | Father |
Mean | ||||
|---|---|---|---|---|---|---|---|
| B5 | B12 | B14 | B25 | B50 | |||
| 2012 | B5 | NT | NT | 31.1 ± 2.8 | NT | 35.0 ± 2.7 | 33.0 ± 1.9 |
| B12 | 6.4 ± 2.7 | NT | Failed | Failed | NT | ||
| B14 | 9.8 ± 2.8 | NT | NT | NT | 2.9 ± 2.7 | ||
| B25 | NT | Failed | NT | NT | 6.7 ± 3.8 | ||
| B50 | 7.5 ± 3.8 | NT | NT | 10.5 ± 2.7 | NT | 9.0 ± 2.3 | |
| 2017 | B5 | NT | 27.6 ± 6.1 | 5.3 ± 5.1 | NT | 14.3 ± 6.4 | 15.7 ± 3.5 ab |
| B12 | 27.8 ± 4.4 | NT | 12.5 ± 4.2 | 17.2 ± 4.2 | NT | 19.2 ± 2.5 a | |
| B14 | 6.1 ± 5.1 | 16.1 ± 4.2 | NT | NT | 6.3 ± 4.2 | 9.5 ± 2.6 b | |
| B25 | NT | 12.5 ± 6.0 | NT | NT | 10.4 ± 4.2 | 11.5 ± 3.7 ab | |
| B50 | NT | 7.8 ± 4.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | NT | 2.6 ± 3.0 c | |
Data for each year are means ± standard error of 10 replicates with 6–10 explants each. For data from the 2017 experiment, values followed by the same letter were not different according to the Mann–Whitney U-test. NT, not tested.