Table 4.

Effect of incubation temperature and mother tree on the frequency (%) of maritime pine megagametophytes that produced established EMs after 90 days in culture.

Mother tree	Incubation temperature
	18°C	23°C	28°C
1007	0.0 ± 0.0 b	9.8 ± 2.1 a	4.3 ± 2.1 a
1046	0.0 ± 0.0 b	4.8 ± 2.0 a	4.3 ± 1.9 a
1058	0.0 ± 0.0 b	10.9 ± 5.5 a	20.8 ± 7.2 a

Data are means ± standard error of 20 replicates with 6–10 explants each. For each mother tree, values followed by the same letter were not different according to the Mann–Whitney U-test.