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. 2019 Feb 19;9:28. doi: 10.3389/fcimb.2019.00028

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Copyright © 2019 Nearing, Connors, Whitehouse, Van Limbergen, Macdonald, Kulkarni and Langille.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prevalence of genes associated with virulence factors is significantly higher in samples from IC patients. A higher proportion of metagenomic shotgun sequencing reads were mapped to the Virulence Factor Database (A) in IC patients (p = 0.02). Proportion of metagenomic shotgun sequencing reads mapped to the Comprehensive Antibiotic Resistance Database (B) (p = 0.072). Differential prevalence was tested using a Wilcoxon rank sum test with an alpha value of 0.05 (represented by *). Points colored by patient (Supplementary Figure 1).