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. 2019 Feb 19;10:269. doi: 10.3389/fimmu.2019.00269

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Copyright © 2019 Terrinoni, Holmgren, Lebens and Larena.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Absence of NFκB signaling impairs systemic and mucosal antibody responses after intragastric immunization with ovalbumin (OVA) with or without cholera toxin (CT). B6 [wild-type (WT)] or NFκB^−/− mice were immunized twice orally with 1 mg OVA together with or without 10 μg CT at an interval of 10 days. Levels of anti-OVA IgG in sera (A), and anti-OVA IgA in intestinal tissue extracts (B), or fecal extracts (C) 10 days after the last immunization were measured by ELISA. The data presented are pooled from two independent experiments showing similar results. ^*represents p < 0.05 for indicated comparisons.