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. 2019 Feb 14;25(2):103–113. doi: 10.1089/ten.tec.2018.0351

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© Claire Crowley et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — Purification of Luc-ZsGreen⁺ primary hMABs after effective transduction with ZsGreen-Luciferase lentiviral vectors. (A) Representative images of hMABs before (ZsGreen-Luc⁻) and after (ZsGreen-Luc⁺) transduction with lentivirus carrying the pHIV-Luc-ZsGreen vector (brightfield and ZsGreen fluorescence). (B) Transduction efficiency, determined by FACS analysis as a percentage of ZsGreen⁺ cells, using the MOI of 7.2, associated to the highest transduction efficiency, 84.6%. (C) ZsGreen-Luc⁺ cells were then sorted based on the expression of ZsGreen to obtain a purified population for seeding experiments. hMAB, human mesoangioblast; FACS, fluorescence-activated cell sorting; MOI, multiplicity of infections. Color images are available online.