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. 2019 Feb 14;25(2):103–113. doi: 10.1089/ten.tec.2018.0351

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© Claire Crowley et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 5. — Bioluminescence imaging of 3D hMAB-seeded scaffolds in vivo. (A) Unseeded and Luc-ZsGreen⁺ hMAB-seeded scaffolds were implanted subcutaneously in the flank of NSG mice. (B) Graph of bioluminescence average radiance shows quantification of transplanted cells during the 7-day in vivo analysis. Data: mean ± SEM (n = 3). (C) Daily bioluminescence imaging of NSG mice with implanted unseeded and Luc-ZsGreen⁺ cell-seeded scaffolds. (D–G). (D) H&E, (E) representative images of immunohistochemistry using STEM121, (F) anti-luciferase, and (G) ZsGreen epifluorescence to demonstrate the presence of Luc-ZsGreen⁺ hMABs in cell-seeded scaffolds after 7 days in vivo, while no signal was detected in unseeded control scaffolds. NSG, NOD-SCID gamma. Color images are available online.