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LC-MS quantification of total glycosaminoglycans from cells collected on days 3, 7, and 21 of hBMSC differentiation. Changes are compared to undifferentiated hBMSCs (CTR) at each time point. Data presented as average ± SD (n = 3). * and # indicate statistically significant changes of P < 0.05 and P < 0.003, respectively. LC-MS, liquid chromatography–mass spectrometry.
