Figure 8.

In Vitro Clonogenic Assay

Data are represented as number of colonies in BCPAP cells, K1 cells, and BHP 2-7 cells treated with 0.05 μM panobinostat and 0.1 μM dabrafenib/2.5 μM selumetinib alone or in combination with (¹³¹I+) or without (¹³¹I−) ¹³¹I. The numbers of colonies in BRAF^V600E-mutant cells decreased significantly after radioiodine treatment when pretreated with panobinostat or MAPKi (dabrafenib or selumetinib) compared to DMSO-treated controls. Compared with HDACi-treated groups, the numbers of colonies further reduced in BRAF^V600E-mutant cells when combined therapies were performed. The numbers of BHP 2-7 cell colonies were significantly reduced after radioiodine treatment when pretreated with HDAC inhibitor alone, which were not further amplified when added with MAPKi. Data are expressed as mean ± SD. *p < 0.05 compared with DMSO-treated cells. **p < 0.05 for comparison with the panobinostat-treated group. Pa, panobinostat; Da, dabrafenib; Se, selumetinib.