Table 3.
Primer sets used for cloning and qRT-PCR of TaGW8-B1 gene in this study.
| Primer | Primer sequence (5′-3′) | Annealing Temp. (°C) | Size of PCR fragment (bp) |
|---|---|---|---|
| TaGW8-P1 | Forward: CCAACACACAAGCTCCAGAC Reverse: GCAACACATGAACAGCCACT |
58 | 1433 |
| TaGW8-P2 | Forward: GAATGAGAGGGACAGGGGAG Reverse: TCATGAATGGGGCCAGGATT |
57 | 1151 |
| TaGW8-P3 | Forward: TCTCTTCGCTCATCCATTCCT Reverse: GCTATCCCCACAACATAGACC |
54 | 1297 |
| TaGW8-P4 | Forward: ATGCAGTGTGGACTTTGTGG Reverse: TTGCGATGCCAGTTGAACAT |
57 | 1149 |
| TaGW8-P5 | Forward: TCCTCTACTCGGAACCTGAA Reverse: GTGCCCATCAAGACGCTTTC |
55 | 1235 |
| TaGW8-P6 | Forward: TGTCTAACTACCTAGGAGCATCT Reverse: TGTGAAGAAGGTAAAGAGTGGC |
61 | 1073 |
| TaGW8-P7 | Forward: CTTGCAGCTTGTAACCCACC Reverse: GAACGTCGGTGCTCTGAAAT |
61 | 1498 |
| GW8-7B | Forward: CGCTCATCCATTCCTTCATCG Reverse: GCTATATGGGTTGGTGTCGC |
60 | 1097/1373 |
| TaGW8-P8 | Forward: TTCTCTGATGGGCTGACTCC Reverse: TGGGGATGTGTTCAGTCTGT |
60 | 121 |
| β-actin gene primer | Forward: GTGTCGCACCAGAGGATCAT Reverse: CGCTGGCATACAAGGACAGA |
60 | 153 |