Table 1.

Binding/inhibition constants derived from isothermal analysis of DSF and from enzyme activity assays.

Interaction (ligand/protein)	Kd (µM), from DSF	Ki (µM), from enzyme assay
SULFA/h-CA I	1134 ± 106	786 ± 60
SULFA/b-CA II	106 ± 9	136 ± 5
ACTAZ/h-CA I	7.2 ± 0.8	2.1 ± 0.2
ACTAZ/b-CA II	<0.5	0.48 ± 0.03
METAZ/h-CA I	1.2 ± 0.1	1.9 ± 0.2
METAZ/b-CA II	0.35 ± 0.03	0.52 ± 0.03
TFMSA/h-CA I	<0.5	<1
TFMSA/b-CA II	<0.5	0.12 ± 0.04