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. 2019 Feb 25;10:931. doi: 10.1038/s41467-019-08862-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cep57 is an evolutionarily conserved component and forms ring-like structures around the mother centriole wall. a Schematic diagrams of H. sapiens Cep57 (HsCep57) and T. cruzi Cep57 (TcCep57). Coiled-coil domains are shown in gray box. The position of evolutionarily conserved domain (PINC motif) is indicated in pink line. b Alignments of the evolutionarily conserved domain (PINC motif) within H. sapiens, M. musculus, G. domesticus, X. laevis, D. rerio, and T. cruzi. Identical residues determined by Clustal Omega are shown in red. Asterisks indicate the residues identical in all aligned sequences; colons: conserved substitutions; periods: semi-conserved substitutions. c Centriolar distribution of Cep57 at different cell cycle stages. HeLa cells were immunostained with antibodies against Cep57 (red) and GT335 (green) and observed by STED microscopy. Scale bar, 1 μm. d STED images representing top views of Cep57 and Cep192 at mother centrioles. Scale bar, 200 nm. e The graph shows radial profiles from the center of the Cep57 and Cep192 rings. The obtained profile was then fitted with a Gaussian curve and the distance between the center of the ring and the peak of the Gaussian curve was defined as the radius. The diameter of the ring was defined as twice the radius. Values are mean distance ± s.d. (n = 10). f Cep57 becomes gradually enriched at new mother centrioles in interphase. HeLa cells were immunostained with antibodies against Cep57 and centrin-2. Arrowheads indicate new mother centrioles (OM old mother, NM new mother). Scale bar, 1 μm. g The signal intensity of Cep57 at new mother centrioles is proportional to that of PCNT. HeLa cells were immunostained with antibodies against Cep57 and PCNT. Scale bar, 1 μm. h Dot plots represent quantification of the signal intensity of Cep57 and PCNT at new mother centrioles (n = 36). i HeLa cells co-expressing HA empty (control), HA-Cep57, or the indicated HA-Cep57 deletion mutants (1 μg) and Histone 2B-RFP (transfection control, 10 ng) were immunostained with antibodies against HA (green), Cep192 (cyan) and RFP (red). Scale bar, 5 μm in the low-magnified view, 1 μm in the inset