Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 25;10:931. doi: 10.1038/s41467-019-08862-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — The binding of PCNT to Cep57 at centrioles is required for the proper localization pattern of PCNT to organize mitotic PCM. a Schematic of PCNT and the ΔPACT mutant or MOPD2 disease-related mutants used for co-IP assays. Coiled-coil domains are shown in gray box and the PACT domain in blue box. The region of PCNT for Cep57-binding is represented in red. The right columns show a summary of the co-IP results and rescue experiments, respectively. b HEK293T cells co-expressing HA-Cep57 and FLAG-PCNT full-length or the indicated mutants were IPed with FLAG antibodies. c The signal intensity of HA-Cep57 bands in b was quantified. Values are mean ± s.d. from three independent experiments. Tukey’s multiple comparisons test was used to obtain p value. *p < 0.05; **p < 0.01; NS not significantly different (p > 0.05). d HeLa cells were treated with siControl or siPCNT, followed by transfection with FLAG empty (control), RNAi-resistant (RNAi-R) PCNT or the indicated mutants. The cells were immunostained with antibodies against FLAG (green), Cep192 (cyan) and CP110 (red). e Histograms represent frequency of mitotic cells with the indicated phenotypes observed in d in each condition. Values are mean percentages ± s.d. from three independent experiments (n = 50 for each experiment). f Schematic of PCNT and the PCNT-Cep57 chimera mutant used for PCNT knockdown and rescue experiments. g HeLa cells were treated with siControl or siPCNT, followed by transfection with FLAG empty (control), RNAi-resistant PCNT, or the PCNT-Cep57 chimera mutant. The cells were immunostained with antibodies against FLAG (green), Cep192 (cyan), and CP110 (red). h Histograms represent frequency of mitotic cells with the indicated phenotypes observed in g in each condition. Values are mean percentages ± s.d. from three independent experiments (n = 50 for each experiment). All scale bars, 5 μm in the low-magnified view, 1 μm in the inset. Left–right arrows indicate precociously disengaged centrioles. Tukey’s multiple comparisons test was used in e and h to obtain p value. *p < 0.05; **p < 0.01; NS not significantly different (p > 0.05)