Fig. 2.

Specific posttranscriptional modifications are underrepresented in Ribo-T assembly intermediates. a Sedimentation profiles (A₂₅₄) of wt ribosomes and Ribo-T. The light shoulder (gray) and heavy shoulder (orange) material of the Ribo-T peak were collected and analyzed separately. b The presence of PTMs in Ribo-T rRNA relative to the wt rRNA. The small subunit PTMs are marked in red, the large subunit PTMs are shown in blue. PTMs present in the LS material at a ≤0.6 level in comparison with the mature wt ribosomes are indicated with the asterisks. Error bars represent deviation from the mean (n = 4). c The location of the under-modified residues in the small (left) and large (right) subunits of Ribo-T. P-site tRNA (P-tRNA) is blue, A-site tRNA (A-tRNA) is green, and mRNA is colored magenta. Source data for panel b are provided in the Source Data file