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. 2019 Feb 25;9:2711. doi: 10.1038/s41598-019-39077-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SPARC and laminin-α1β1γ1, two proteins abundantly found in EPC-CM, control acinar differentiation. (a) RT-qPCR analysis of acinar and control genes reported to β-actin in pancreatic explants cultured for 3 days in control medium (Ctrl) and in the presence of SPARC (+SPARC). Addition of SPARC favors acinar differentiation as demonstrated by increased levels of acinar markers (Cpa and Amy) and pro-acinar transcription factors (Rbpjl and Ptf1a). Expression level of control genes (Rbpj and E-Cadherin) are not affected. (b) RT-qPCR analysis of acinar and control genes reported to β-actin in pancreatic explants cultured for 3 days in control medium (Ctrl) and in the presence of laminin-α1β1γ1. In the presence of laminin-α1β1γ1, the expression of acinar markers (Cpa and Amy) and pro-acinar transcription factors (Rbpjl and Ptf1a) is reduced as compared to control explants. Expression level of control genes (Rbpjl and E-Cadherin) is not affected. (Mann-Whitney: *p < 0.05). (c) Immunofluorescence for the acinar differentiation marker Amylase (green) and the pancreatic epithelium marker (E-Cadherin, red) in explants at 3 days, as indicated. As compared to control explants showing acinar differentiation (Amylase⁺ cells), addition of SPARC stimulates, while laminin-α1β1γ1 decreases the Amylase signal.