Table 3.
List of antibodies used in the Western blot analyses, immunofluorescence and immunohistochemistry.
| Antibody name | Catalog number | Vendor name and location | Concentration Immunofluorescence /Western blot |
|---|---|---|---|
| GAPDH (loading control) | 5174 | CST, Boston, MA, USA | −/1:1000 |
| E-cadherin | 3195 S | CST | 1:100/1:1000 |
| Vimentin | ab8978 | Abcam, Cambridge, UK | 1:100 |
| Alpha smooth muscle actin (α-SMA) | ab5694 | Abcam | 1:100 |
| F-actin | ab205 | Abcam | 1:100 |
| Oxytocin receptor (OTR) | ab115664 | Abcam | 1:200 |
| Desmin | ab6322 | Abcam | 1:200 |
| Smooth muscle, myosin heavy chain (SM-MHC) | ab81031 | Abcam | 1:200 |
| Neurokinin 1 receptor (NK1R) | ab183713 | Abcam | 1:50 |
| Calcitonin receptor like receptor (CRLR) | ab84467 | Abcam | 1:200 |
| Receptor activity modifying protein 1 (RAMP-1) | ab203282 | Abcam | 1:100 |
| Calcitonin gene related peptide (CGRP) | ab47027 | Abcam | 1:200 |