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. 2019 Feb 25;9:2728. doi: 10.1038/s41598-019-39565-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HtrA4 inhibits HUVEC proliferation. HUVECs were treated with vehicle control or two doses of HtrA4 over 48 h. (a) Growth curve. (b) Percentage of viable cells. (c) Immunofluorescence of cell proliferation marker Ki67 at 24 h, representative images at 10x magnification are shown. (d) Quantification of Ki67 staining of (c). n = 3. Data is expressed as mean ± SD, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.