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. 2019 Feb 25;9:2728. doi: 10.1038/s41598-019-39565-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HtrA4 disrupts tube formation in EPCs. Cells were treated with vehicle control or two concentrations of HtrA4, and their abilities to form tubes were assessed. Four independent EPC clones were examined. (a) Representative images at 4x magnification. (b–d) Quantification of tube formation. (b) Total tube length per mm² area, (c) Total branching point per mm² area, and (d) Number of tubules per mm² area. n = 4 for all. Data are expressed as mean ± SD. ^**p < 0.01, ^***p < 0.001.