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. 2019 Feb 25;9:2679. doi: 10.1038/s41598-019-39093-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular weight determination of the by-products by gel permeation chromatography against a Hp calibrate. Average molecular weight (Mw) suggested no correlation with purity, however, the polydispersity (Pd = Mw/Mn)) was reduced up to and including the removal of Hp. The loss of sample below 100% in the detected range was due to the resolution range residing between 4 and above 40 kDa (Hp – 99.7%, A – 94.3%, B – 90.3%, C – 94.4%, D – 98.1%). *Mn – number average molecular weight. Average molecular weight (kDa) – Hp, 16.6, A, 30.6, B, 18.7, C, 33.5, D, 26.3. Pd – Hp, 1.2, A, 2.2, B, 2.0, C, 2.1, D, 1.7.