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. 2019 Feb 25;9:2679. doi: 10.1038/s41598-019-39093-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of BaF3-1c cells was induced by Hp or by-products in response to FGF2. Cells were incubated in the presence of 0.1 nM FGF2 and a range of by-product samples or Hp control (1–100 µg/ml) for 72 hours. Proliferation was determined by MTT assay, reading the absorbance at 570 nm (n = triplicate of 3 biological repeats). The mean and standard error for each sample was calculated and multiple t-tests (p = 0.05) were carried out for all repeats, at all concentrations and samples were compared to Hp. P-values with a significant increase over Hp – 100 µg/ml A, 0.002, B, <0.0001, C, 0.002, D, <0.0001, 30 µg/ml A, 0.005, B, <0.0001, C, 0.0006, D, <0.0001, 10 µg/ml D, 0.01. P-values with a significant decrease below Hp – 3 µg/ml A, <0.0001, B, 0.002.