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. 2018 Dec 21;19(3):1654–1664. doi: 10.3892/mmr.2018.9785

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — EPDMNQ and ENDMNQ induce apoptosis in hepatocellular carcinoma cells. (A) Hep3B cells were treated with 4 µmol/l 5-FU, EPDMNQ or ENDMNQ for different time intervals (3, 6, 12 or 24 h) and stained with Annexin V-FITC/PI. Images represent fluorescence microscopic images (original magnifications, ×400). (B) Quantification of fluorescence intensities from A. (C) Apoptosis distribution was determined by flow cytometry following treatment with 4 µmol/l 5-FU, EPDMNQ or ENDMNQ for different time intervals (3, 6, 12 or 24 h). (D) Quantification of flow cytometry from C. Data are expressed as the percentage of viable cells. ***P<0.001 vs. 5-FU group. EPDMNQ, 2,3-dihydro-2,3-epoxy-2-propylsulfonyl-5,8-dimethoxy-1,4-naphthoquinone; ENDMNQ, 2,3-dihydro-2,3-epoxy-2-nonylsulfonyl-5,8-dimethoxy-1,4-naphthoquinone; 5-FU, 5-fluorouracil; FITC, fluorescein isothiocyanate; PI, propidium iodide.