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. 2019 Jan 22;19(3):2164–2172. doi: 10.3892/mmr.2019.9883

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — GAS5 expression levels following the transduction of NPCs with lentivirus containing GAS5 lncRNA. (A) Efficiency obtained 96 h following transduction. Magnification, ×40. (B) Protuberances of NPCs observed during cell growth. Magnification, ×100. (C) GAS5 expression levels assessed by reverse transcription-quantitative polymerase chain reaction at 96 h following transduction. Data are presented mean ± standard deviation from three independent experiments performed in triplicate. *P<0.05 vs. NC group. GAS5, growth arrest-specific transcript 5; lncRNA, long noncoding RNA; NC, negative control; NPCs, nucleus pulposus cells; OE, overexpression.