Figure 3.

Cellular responses induced in mice after three immunizations with β-Gal co-administered with the adjuvant system c-di-AMP/alum via intramuscular route. Splenocytes from vaccinated animals (n = 10) were restimulated with an increased concentration of β-Gal protein (starting from 1 μg/ml to 40 μg/ml). Proliferation of the cells after 96 h was indirectly determined by counting [³H]-thymidine events incorporated into the DNA of proliferating cells. Results are average of quadruplicates and are expressed as stimulation index (SI). Statistically significant differences with respect to β-Gal alone (*), β-Gal with alum (o) or β-Gal with c-di-AMP (+) were validated using one-way ANOVA with Tukey's post hoc test, with p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).