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. 2019 Feb 19;9:31. doi: 10.3389/fcimb.2019.00031

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Copyright © 2019 Ebensen, Delandre, Prochnow, Guzmán and Schulze.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine profiles stimulated by β-Gal co-formulated with different adjuvants. The presence of mouse IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17A, IL-22, IFN-γ, and TNF-α were determined in an immunoassay using a cytometric bead array according to the manufacturer's instructions (Mouse Th1/Th2/Th9/Th17 13plex Biolegend). Results are presented as a heat map of Th1, Th2, pro-inflammatory, and Th17 cytokines in antigen-restimulated splenocytes derived from mice (n = 10) vaccinated with β-Gal co-administered with alum, c-di-AMP or c-di-AMP/alum.