Figure 4.

Effects of DPL extract on the degradation or phosphorylation of NF-κB/p65 and IκB-α protein in LPS-induced RAW264.7 cells. RAW264.7 cells were treated with LPS (1 µg/ml) in the presence or absence of DPL extract at the indicated concentrations for 24 h. NF-κB phosphorylation and IκB-α degradation were assessed by western blotting. Data analysis was performed using ImageJ software by measuring the integrated band densities following background subtraction. Each bar represents the mean ± standard deviation calculated from three independent experiments. *P<0.05 vs. LPS only treatment. DPL, Dendropanax morbifera leaf; LPS, lipopolysaccharide; NF-κB, nuclear factor-κB; IκB-α, NF-κB inhibitor-α; p-, phosphorylated.