Table I.
Primers used in the present study.
| Gene | Primer | Annealing temperature, °C | Product size, bp |
|---|---|---|---|
| GAPDH | 58 | 163 | |
| F | 5′-CACATGGCCTCCAAGGAGTA-3 | ||
| R | 5′-TCCCCTCTTCAAGGGGTCTA-3′ | 58 | |
| PCSK9 | 58 | 140 | |
| F | 5′-TGGAACTCACTCACTCTGGG-3′ | ||
| R | 5′-AAGAATCCTGCCTCCTTGGT-3′ | 58 | |
| Bax | 58 | 119 | |
| F | 5′-TGATCAGAACCATCATGGGC-3′ | ||
| R | 5′-GGACATCAGTCGCTTCAGTG-3′ | 58 | |
| Bcl-2 | 58 | 156 | |
| F | 5′-GAAGAAGCCACCCTCAAGC-3′ | 58 | |
| R | 5′-AGCAAGGACACCCGCACTC-3′ | ||
| Caspase-3 | 58 | 106 | |
| F | 5′-GAGGCCGACTTCTTGTATGC-3′ | ||
| R | 5′-GTTTCAGCATGGCACAAAGC-3′ | 58 | |
| NF-κB | 58 | 186 | |
| F | 5′-AGACAAATGGGCTACACCGA-3′ | ||
| R | 5′-AAAGCTGAGTTTGCGGAAGG-3′ | 58 |
F, forward; R, reverse; PCSK9, proprotein convertase subtilisin/kexin type 9; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2 associated X, apoptosis regulator; NF-κB, nuclear factor-κB.