Table II.
Antibody details.
| Primary antibody | Secondary antibody | |||||||
|---|---|---|---|---|---|---|---|---|
| Antibody | Supplier | Catalog no. | Dilution | Species raised | Supplier | Catalog no. | Dilution | Molecular weight, kDa |
| PCSK9 | Sigma-Aldrich | SAB1302902 | 1:500 | Rabbit | Abcam | ab191866 | 1:2,000 | 72 |
| Caspase-3 | Abcam | ab32042 | 1:100 | Rabbit | Abcam | ab191866 | 1:5,000 | 32 |
| Bax | Abcam | ab32503 | 1:5,000 | Rabbit | Abcam | ab191866 | 1:10,000 | 21 |
| Bcl-2 | Abcam | ab32124 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:5,000 | 26 |
| p38 | Abcam | ab27986 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:10,000 | 38 |
| p-p38 | Abcam | ab4822 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:10,000 | 38 |
| ERK | Abcam | ab17942 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:5,000 | 42-44 |
| p-ERK | Abcam | ab214362 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:5,000 | 42-44 |
| JNK | Abcam | ab179461 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:5,000 | 46 |
| p-JNK | Abcam | ab124956 | 1:1,000 | Rabbit | Abcam | ab191866 | 1:5,000 | 46 |
| β-actin | Abcam | ab8226 | 1:10,000 | Mouse | Abcam | ab131368 | 1:10,000 | 43 |
Sigma-Aldrich; Merck KGaA Darmstadt, Germany and Abcam, Cambridge, UK. PCSK9, proprotein convertase subtilisin/kexin type 9; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2 associated X, apoptosis regulator; p-, phosphorylated; ERK, extracellular signal-regulated kinases; JNK, c-Jun N-terminal kinases.