Table III.
Summary of Vγ9δ2 T cells harvested under four different culture conditions and recommended application for the culture methods.
| Group | a | b | c | d |
|---|---|---|---|---|
| Initial seeding density (×105 cells) | 2.00 | 2.00 | 2.00 | 2.00 |
| Day 4 | ||||
| Total cell count (×105 cells) | 5.50±0.50 | 1.78±0.38 | 5.73±0.08 | 3.99±0.16 |
| Vγ9δ2 T cell (%) | 28.25±1.55 | 4.02±0.17 | 24±6.00 | 8.10±5.21 |
| Vγ9δ2 T cell count (×105 cells) | 1.55±0.06 | 0.07±0.02 | 1.38±0.33 | 0.31±0.19 |
| Day 16 | ||||
| Total cell count (×105 cells) | 15.8±1.10 | 33.00±1.00 | 9.90±0.80 | 16.40±4.40 |
| Vγ9δ2 T cell (%) | 47.75±6.76 | 55.15±4.39 | 52.50±18.09 | 53.25±4.40 |
| Vγ9δ2 T cell count (×105 cells) | 7.52±0.40 | 18.18±0.40 | 5.18±1.21 | 8.77±0.75 |
| Application | Short-term culture | Long-term culture | Unsuitable | Unsuitable |