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Primers and probes for reverse transcription-quantitative polymerase chain reaction.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Probe (5′FAM, 3′BHQ-1) |
|---|---|---|---|
| flTF | TGATGTGGATAAAGGAGAAAACTACTGT | CTACCGGGCTGTCTGTACTCTTC | TTCAAGCAGTGATTCCCTCCCGAACA |
| asTF | ATCTTCAAGTTCAGGAAAGAAATATTCTAC | GCTCTGCCCCACTCCTGCC | TTGGAGCTGTGGTATTTGTGGTCATCATC |
| GAPDH | CAGGGCTGCTTTTAACTCTGGT | CATGGGTGGAATCATATTGGAAC | TGGATATTGTTGCCATCAATGA CCCCT |
asTF, alternatively spliced TF; flTF, full-length TF; TF, tissue factor.
